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2 edition of Identification of an intervening sequence within the 23S rRNA gene of Coxiella burnetii found in the catalog.

Identification of an intervening sequence within the 23S rRNA gene of Coxiella burnetii

Robert Guy Afseth

Identification of an intervening sequence within the 23S rRNA gene of Coxiella burnetii

by Robert Guy Afseth

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  • 37 Currently reading

Published .
Written in English

    Subjects:
  • Coxiella burnetii -- Genetics.

  • Edition Notes

    Statementby Robert Guy Afseth.
    The Physical Object
    Paginationviii, 46 leaves, bound :
    Number of Pages46
    ID Numbers
    Open LibraryOL17003132M

    Intervening sequences (IVSs) were originally identified in the rrl genes for 23S rRNA (rrl genes, for large ribosomal subunit, part of rrn operon encoding rRNA) of Salmonella enterica serovars Typhimurium LT2 and Arizonae. These sequences are transcribed but later removed during RNase III processing of the rRNA, resulting in fragmentation of the 23S species; IVSs are uncommon, but have been. the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA. J Bacteriol ; 5. Weisburg WG, et al. Phylogenetic diversity of the rickettsiae. J Bacteriol ; 6. Thiele D, Willems H, Krauss, H. The 16S/23S ribo-somal spacer region of Coxiella burnetii. Eur J.

    Coxiella burnetii is an obligate intracellular bacterium with a doubling time of 5–7 hours. Chromosomal DNA from C. burnetii was digested with various restriction enzymes previously determined to not cut within the genomic 16S rRNA gene, or with a combination of these noncutting enzymes in conjunction with AflIII, a restriction enzyme that cuts twice within the 16S rRNA gene.   G. Afseth, Y-Y. Mo, L.P. MallaviaCharacterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene J. Bacteriol, (), pp.

      Intervening sequences that lead to rRNA fragmentation are highly variable in sequence and length an IVS with a similar ORF was found at the same position in the gamma‐Proteobacterium Coxiella burnetii The sequences flanking 16S rRNA, 23S rRNA and the helix 9 IVS of 23S rRNA in alpha‐Proteobacteria are complementary to each other. Intervening sequences (IVSs) occur sporadically in several bacterial genera in the genes for 23S rRNA at relatively conserved locations. They are cleaved after transcription and lead to the presence of fragmented rRNA, which is incorporated into the ribosomes without religation but is nevertheless functional. The fragmentation of rRNA and the number of IVSs in all 72 strains of the Salmonella.


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Identification of an intervening sequence within the 23S rRNA gene of Coxiella burnetii by Robert Guy Afseth Download PDF EPUB FB2

Characterization of the rRNA operon from the obligate intracellular bacterium Coxiella burnetii has determined the order of the rRNA genes to be 16SS-5S. A bp intervening sequence (IVS) was identified to interrupt the 23S rRNA gene beginning at position The IVS is predicted to form a stem-loop structure formed by flanking inverted Cited by: The intervening sequence (IVS) of Coxiella burnetii, the agent of Q fever, is a nt selfish genetic element located in helix 45 of the precursor 23S rRNA.

CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene. The intervening sequence (IVS) of Coxiella burnetii, the agent of Q fever, is a nt selfish genetic element located in helix 45 of the precursor 23S rRNA.

The IVS element, in turn, contains an ORF that encodes a hypothetical ribosomal S23 protein (S23p). Although S23p can be synthesized in vitro in the presence of an engineered E.

coli promoter and ribosome binding site, results suggest Cited by: 5. Characterization of the rRNA operon from the obligate intracellular bacterium Coxiella burnetii has determined the order of the rRNA genes to be 16SS-5S.

A bp intervening sequence (IVS) was identified to interrupt the 23S rRNA gene beginning at position Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene. J Bacteriol. ; –9. [PMC free article] Raghavan R, Miller SR, Hicks LD, Minnick MF.

The unusual 23S rRNA gene of Coxiella burnetii: two self-splicing group I introns flank a base -pair. A previously published sequence of the 23S rRNA gene of Coxiella burnetii has been reported to contain an intervening sequence of base pairs (bp).

The sequence information on the intervening sequence and the 23S rRNA gene was exploited to develop a specific PCR-based assay for C. burnetii. A primer set was designed that amplified a bp fragment encompassing part of the intervening.

We describe the presence and characteristics of two self-splicing group I introns in the sole 23S rRNA gene of Coxiella two group I introns, Cbu.L and Cbu.L, are inserted at sites and (Escherichia coli numbering), respectively, in the 23S rRNA gene of C.

introns were found to be self-splicing in vivo and in vitro even though the terminal nucleotide. Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene. PUBMED EPMC Raghavan R, Miller SR, Hicks LD, Minnick MF;, J Bacteriol.

; The unusual 23S rRNA gene of Coxiella burnetii: two self-splicing group I introns flank a base. cellular arthropod pathogen (23). Various intervening elements have been identified within physiologically vital genes of C. burnetii. A putative excisable intein is located in the C-terminal region of the replicative DNA helicase (CBU), and the sole 23S rRNA gene is interrupted both by an intervening sequence (24) and a group I self-splicing.

Afseth G, Mo YY, Mallavia LP: Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene.

J Bacteriol–9. Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene. Bacteriol. [Europe PMC free article] [Google Scholar]. A previously published sequence of the 23S rRNA gene of Coxiella burnetii has been reported to contain an intervening sequence of base pairs (bp).

G. Afseth, Y.-Y. Mo, L.P. MallaviaCharacterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene J. Bacteriol., (), pp. Afseth G, Mo YY, Mallavia LP. Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene.

J Bacteriol. May; (10)– [PMC free article] Selenska-Pobell S, Evguenieva-Hackenberg E. Fragmentations of the large-subunit rRNA in the family Rhizobiaceae. G. Afseth, Y.Y. Mo, L.P. MallaviaCharacterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene J.

Bacteriol., (), pp. The intervening sequence (IVS) of Coxiella burnetii, the agent of Q fever, is a nt selfish genetic element located in helix 45 of the precursor 23S IVS element, in turn, contains an ORF that encodes a hypothetical ribosomal S23 protein (S23p).

Although S23p can be synthesized in vitro in the presence of an engineered E. coli promoter and ribosome binding site, results suggest that. Certain Campylobacter strains harbor a transcribed intervening sequence (IVS) in their 23S rRNA genes.

Following transcription, the IVS is excised, leading to fragmentation of the 23S rRNA. The origin and possible functions of the IVS are unknown. Furthermore, the distribution of IVS-harboring strains within Campylobacter populations is poorly understood. G. Afseth, Y.Y. Mo, L.P.

MallaviaCharacterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene J Bacteriol, (), pp. Intervening sequences (IVSs) in the rrl genes for 23S rRNA are transcribed but later removed by RNase III without religation during RNA processing, leading to fragmented rRNA.

We examined about strains of the family Enterobacteriaceae for presence of IVSs using PCR. No IVSs were detected in strains belonging to Escherichia, Shigella, Enterobacter, Erwinia, Ewingella, Hafnia, Kluyvera.

burnetii and Identification of an Intervening Sequence within the 23S rRNA Gene By G Afseth, Y Y Mo, L P Mallavia, J. Bacteriol, Guy Afseth, Yin-yuan Mo and Louis P. Mallavia Abstract.C. burnetii 23S rRNA gene linkage map. Two group 1 introns, Cbu.L and Cbu.L, flank an essential bp 23S rRNA gene exon.

Cbu.L also encodes a putative HE (Cbu_).Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene. J. Bacteriol.